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Image Search Results
Journal: British Journal of Pharmacology
Article Title: Does PKC activation increase the homologous desensitization of μ opioid receptors?
doi: 10.1111/bph.12712
Figure Lengend Snippet: PMA-induced translocation of PKC to the plasma membrane. (A) A fluorescent PKC sensor (upward-DAG) was imaged in Flag-MOP receptor (MOPr) expressing HEK293 cells. Confocal images were taken before (left column) or following 10 min exposure (middle column) to either morphine (15 μM, top), PDBu (0.2 μM, middle) or PMA (1 μM, bottom). Flag-MOP receptors were labelled with M1-Alexa 594 (right column) to identify the plasma membrane and to confirm that Flag-MOP receptors were expressed in individual cells. (B) Summary of upward-DAG fluorescence intensities at the membrane and cytosol and the ratio of membrane/cytosol expressed as % of upward-DAG fluorescence measured before drug treatment. *P < 0.05; **P < 0.01, ***P < 0.001, paired t-test. Scale bar = 20 μm.
Article Snippet: The
Techniques: Translocation Assay, Clinical Proteomics, Membrane, Expressing, Fluorescence